FRET only occurs if…
1) the donor fluorescence emission spectrum overlaps with the acceptor absorbance.
2) the donor and acceptor fluorophores are in close proximity (i.e. 1 - 9nm, which is at the scale of protein size).
3) the transition dipole moments of the donor and acceptor fluorophores are not perpendicular.
Subtechnologies:
Other technologies in this category:
Measurement methods FRETFRET pairs
FRET efficiency
Papers / Reviews FRET
