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Lambert Instruments

 
 
  • FLIM, Fluorescence Lifetime Imaging Microscopy
  • FRET, Fluorescence Resonance Energy Transfer
  • Image intensifier
  • CCD camera sensitivity
  • (Intensified) High speed camera
technologies » FRET, Fluorescence Resonance Energy Transfer » FRET only occurs if…

FRET only occurs if…

1) the donor fluorescence emission spectrum overlaps with the acceptor absorbance.
2) the donor and acceptor fluorophores are in close proximity (i.e. 1 - 9nm, which is at the scale of protein size).
3) the transition dipole moments of the donor and acceptor fluorophores are not perpendicular.

 

Subtechnologies:

Other technologies in this category:

Measurement methods FRET
FRET pairs
FRET efficiency
Papers / Reviews FRET
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Stageplaats -Internship

Stageplaats / Internship

Stage: FLIM systeem
o.a. meetapparatuur karakteriseren, metingen automatiseren, ontwikkeling van nieuwe meetsystemen t.b.v. fluorescentiemicroscopie.

Internship: FLIM system
Characterization of measurement devices, automating measurements, development of new measurement systems etc for fuorescence microscopy.

application
  • Biomolecular interactions
products
  • II18MD, modulated image intensifier
  • LIFA, Lambert Instruments Fluorescence Attachment
downloads
  • Publications LIFA / II18MD
  • Test sites LIFA / II18MD
  • Presentation FRET ratio and FLIM
tutorials
  • Emission
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