• home
  • news
  • products
  • technologies
  • applications
  • agents
  • events

Lambert Instruments

 
 
  • FLIM, Fluorescence Lifetime Imaging Microscopy
  • FRET, Fluorescence Resonance Energy Transfer
  • Image intensifier
  • CCD camera sensitivity
  • (Intensified) High speed camera
technologies » FRET, Fluorescence Resonance Energy Transfer » FRET pairs

FRET pairs

The emission spectrum of the donor fluorophore has to overlap the excitation spectrum (absorbance) of the acceptor fluorophore. Some examples are BFP-YFP, CFP-YFP, GFP-DsRed, GFP-Cy3, GFP-mOrange, YFP-RFP, and Cy3-Cy5.

Browser based calculator to find the critical distance and FRET efficiency with known spectral overlap. 

 

Subtechnologies:

Other technologies in this category:

Measurement methods FRET
FRET efficiency
FRET only occurs if…
Papers / Reviews FRET
« Back

Stageplaats -Internship

Stageplaats / Internship

Stage: FLIM systeem
o.a. meetapparatuur karakteriseren, metingen automatiseren, ontwikkeling van nieuwe meetsystemen t.b.v. fluorescentiemicroscopie.

Internship: FLIM system
Characterization of measurement devices, automating measurements, development of new measurement systems etc for fuorescence microscopy.

application
  • Biomolecular interactions
products
  • II18MD, modulated image intensifier
  • LIFA, Lambert Instruments Fluorescence Attachment
downloads
  • Publications LIFA / II18MD
  • Test sites LIFA / II18MD
  • Presentation FRET ratio and FLIM
tutorials
  • GFP (and family members)
Newsletter
Sign up for the newsletter Sign in Sign out