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Lambert Instruments

 
 
  • FLIM, Fluorescence Lifetime Imaging Microscopy
  • FRET, Fluorescence Resonance Energy Transfer
  • Image intensifier
  • CCD camera sensitivity
  • (Intensified) High speed camera
technologies »CCD camera sensitivity

CCD camera sensitivity

At low light levels standard CCD/CMOS cameras are not sensitive enough to capture useful images. There are ways to increase the sensitivity of such cameras. The first method is to allow the CCD to integrate for much longer times. In order to prevent high background noise, CCD cooling is applied when using long exposure times. A second method is to use an image intensifier to boost the input signal.

Cooled CCD
With longer integration times not only more input signal is collected but also more dark current from the CCD itself. The amount of dark current depends strongly on the temperature and for every 6°C the CCD is cooled down, the noise (dark current) halves. When the CCD is cooled down to -25°C, integration times up to tens of minutes can be applied. This enhances the sensitivity of the camera dramatically.
To improve the cameras signal to noise ratio, the read-out noise is reduced by using a lower read-out speed. These techniques are used in high performance 14 and 16 bits digital cameras.

Intensified CCD with fiber-optic coupling
An image intensifier helps to increase the sensitivity of a camera by amplifying the input light signal before relaying it to the CCD/CMOS sensor of a camera. There are in principal two ways to relay the output image from an image intensifier to a CCD/CMOS sensor. The first is by means of a relay lens. A lens coupling is flexible, but the downside is that a lens coupling has a relatively low efficiency caused by the limited aperture of a lens. A more efficient way is to use a fibre-optic window to transfer the image from the intensifier to the CCD. Such a fibre-optic window contains a large number of small (6-10 micron) individual fibres and is acting as an image guide. By using a tapered fiber optic window a magnification or demagnification can be accomplished. Generally a de-magnification factor is chosen that will best match the CCD/CMOS sensor size to the output diameter of the intensifier.
 
>The advantages of a fiber-optic coupling are:
- low light losses
- intensifier/CCD combination is more compact
- camera design is sturdier
- no optical adjustments needed

 

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application
  • Cell Biology - Microscopy
product
  • Cooled CCD cameras
  • Intensified CCD cameras
tutorials
  • CCD
  • Dark current
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