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Lambert Instruments

 
 
  • FLIM, Fluorescence Lifetime Imaging Microscopy
  • FRET, Fluorescence Resonance Energy Transfer
  • Image intensifier
  • CCD camera sensitivity
  • (Intensified) High speed camera
technologies » FLIM, Fluorescence Lifetime Imaging Microscopy » Measurement methods FLIM » Multiple lifetime measurements

Multiple lifetime measurements

Some fluorophores have a multi exponential decay, consisting of two or more lifetime components. For example, the decay of CFP is bi-exponential.

These multiple lifetime components can be extracted in two ways, both implemented in the LI FLIM software:
1] Multiple frequency measurements
2] Polar plot

 

Other subtechnologies:

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Stageplaats -Internship

Stageplaats / Internship

Stage: FLIM systeem
o.a. meetapparatuur karakteriseren, metingen automatiseren, ontwikkeling van nieuwe meetsystemen t.b.v. fluorescentiemicroscopie.

Internship: FLIM system
Characterization of measurement devices, automating measurements, development of new measurement systems etc for fuorescence microscopy.

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  • Presentation FLIM applications
tutorials
  • Mono-exponential
  • Multi-exponential
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