Lambert Instruments

For doing fluorescence lifetime imaging microscopy -FLIM we offer the complete system LIFA -Lambert Instruments Fluorescence lifetime imaging Attachment allowing fluorescence image acquisition and the generation of lifetime images. Additionally the key components of this system, the modulated image intensifier II18MD and the modulated LED light source, are offered as separate products for users who decide to build their own FLIM -fluorescence lifetime imaging microscopy system including their own software.

The picture shows a HeLa cell with FRET (Alexa488 and Alexa568) only at the centrosomes (the microtubule organising centres of the cell): the lifetime of Alexa488 at the centrosomes has decreased to 2,3ns, and the lifetime of Alexa488 outside the centrosomes is 2,7ns. Both the lifetime and the intensity image is shown, with the merge at the bottom. Courtesy of Prof. Diaspro, Genua, Italy.