Fluorescence Lifetime Imaging -FLIM

For doing fluorescence lifetime imaging microscopy -FLIM we offer the complete system LIFA -Lambert Instruments Fluorescence lifetime imaging Attachment allowing fluorescence image acquisition and the generation of lifetime images. Additionally the key components of this system, the modulated image intensifier II18MD and the modulated LED light source, are offered as separate products for users who decide to build their own FLIM -fluorescence lifetime imaging microscopy system including their own software.
The picture shows a HeLa cell with FRET (Alexa488 and Alexa568) only at the centrosomes (the microtubule organising centres of the cell): the lifetime of Alexa488 at the centrosomes has decreased to 2,3ns, and the lifetime of Alexa488 outside the centrosomes is 2,7ns. Both the lifetime and the intensity image is shown, with the merge at the bottom. Courtesy of Prof. Diaspro, Genua, Italy.
products
LIFA, Lambert Instruments Fluorescence Attachment

The LIFA -Lambert Instruments Fluorescence Attachment for lifetime imaging microscopy can be attached to any widefield fluorescence microscope and works in the frequency domain, which means that it uses a modulated light source and a modulated image intensifier as detector. The system is easy to install (within 1 hour) and to operate and does not require an optical bench with laser. The LIFA -Lambert Instruments Fluorescence Attachment system has been judged "easy and highly quantitative". And last but not least, it's reasonable priced.
Confocal or TIRF -Total Internal Reflection Fluorescence LIFA

II18MD, modulated image intensifier

The II18MD is an Image Intensifier with an 18mm diameter input screen that can be Modulated in the range of 10-120 MHz and is Digital controlled. The II18MD modulated image intensifier is used as key component for FLIM -Fluorescence Lifetime Imaging Microscopy in the frequency domain. The II18MD modulated image intensifier is designed for users who wish to apply the highest quality detector as part of their set-up for fluorescence lifetime imaging.
Spectrally resolved LIFA
Modulated LED

The Modulated LED light source can be used as the excitation light source in FLIM –fluorescence lifetime imaging microscopy systems that operate in the frequency domain. Modulated LEDs of different wavelength bands are available. The output light intensity can be varied over a wide range and the maximum excitation power available at the sample is comparable with that of other light sources.
Time-resolved fluorometer

Alba system for confocal imaging

The ISS Alba imaging workstation is a computer-controlled instrument specifically designed for confocal imaging applications, by using scanning mirrors or a piezo-controlled xyz stage. The modular design of the instrument allows it to be easily upgraded to acquire Fluorescence Lifetime data (FLIM) and Fluorescence Correlation Spectroscopy (FCS) data.