Lambert Instruments

Typically, the LIFA -Lambert Instruments Fluorescence Attachment is a widefield FLIM -fluorescence lifetime imaging microscopy system with a modulated LED light source. The detector is a modulated ICCD and generates 2D images. However, by placing a spectrograph in the light path just prior to the detector the emission light of one line of the image is spectrally resolved providing extra information about the fluorescence. An image is generated with the fluorescence intensity profile at the y-axis and the emission wavelength at the x-axis. The pseudo colored map indicates the fluorescence lifetime. Look here for a spectrally resolved fluorescence lifetime image.

Spectrally resolved LIFA system:

At the camera port of the microscope, the spectrograph is mounted just prior to the modulated intensified camera of the LIFA -Lambert Instruments Fluorescence Attachment.