Lambert Instruments

The ISS Alba imaging workstation is a computer-controlled instrument specifically designed for confocal imaging applications, by using scanning mirrors or a piezo-controlled xyz stage. The modular design of the instrument allows it to be easily upgraded to acquire Fluorescence Lifetime data (FLIM) and Fluorescence Correlation Spectroscopy (FCS) data.

Lambert Instruments is the distributor of ISS in the BeNeLux and Denmark. More information on ISS products can be found here.

FCS
In confocal Fluorescence Correlation Spectroscopy, the temporal fluctuations of the detected fluorescence signals are recorded. These are the time-dependent spontaneous intensity fluctuations of the fluorescence signal in the observation volume. As the intensity fluctuations in the volume under observation may arise from diffusive Brownian motion, random flow patterns, and chemical reactions, the data stream contains information about these processes occurring at the molecular scale. The fluctuations can be recorded as FCS or as Photon Counting Histogram (PCH), the former technique providing information on the characteristic diffusion times of the molecule and their number, the triplet state function, the size of the excitation volume; while the latter technique recording the brightness of the molecular species and their number.