Lambert Instruments

The Netherlands, 21-11-2006

The FLIM system LIFA-Lambert Instruments Fluorescence Attachment can obtain confocal lifetime images from now on!

In this picture the LIFA-Lambert Instruments Fluorescence Attachment is attached to a fluorescence widefield microscope. The CSU10 spinning disk (Perkin Elmer / Yokogawa) is attached to the camera port of the microscope to obtain confocal images. As detector, the intensified CCD camera of the LIFA-Lambert Instruments Fluorescence Attachment is used, mounted to the camera port of the CSU10 spinning disk. In this set-up, the light source is not the modulated LED as is usually used, but instead the modulated laser diode of 470nm (PicoQuant). The rest of the set-up is exactly the LIFA-Lambert Instruments Fluorescence Attachment (same signal generator box, software, etc).

The confocal scanning method of this spinning disk is based on the Nipkow disk scanner: an optical scanner using rotation of a disk with pinholes to produce an image. By placing a microlens array in front of the Nipkow disk, the optical efficiency is improved about two orders. More details about the technology can be found at the website of Yokogawa.

In the next image two z-positions are taken for the same sample (pollen grain). The acquisition was done with 12 phase steps of 50 msec exposure time at an MCP voltage of 690V.

         
These two images are presented as a merge of the fluorescence lifetime in pseudo colours and the intensity in grey scale.

At the "Najaarsvergadering 2006", Joint Meeting of the NVvM, EMS and BVM in Lunteren (The Netherlands), we will present this new combination of the LIFA-Lambert Instruments Fluorescence Attachment with the spinning disk and modulated laserdiode light source. We welcome you to visit our stand at this exhibition!

Lambert Instruments
www.lambert-instruments.com
0031 (0)50 501 84 61

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