FLIM-Fluorescence Lifetime Imaging Microscopy is the new tool of Lambert Instruments to measure FRET-Fluorescence Resonance Energy Transfer, ion imaging, oxygen imaging, probing microenvironment, and medical diagnosis. This measurement is done quickly and simple with our LIFA-Lambert Instruments-Fluorescence Attachment system attached to a standard widefield fluorescence microscope, upright or inverted.
Is the object you want to image not bright enough or do you need ultra short exposures instead of normal/long exposures? In both cases an image intensifier in front of your camera could solve the problem. You should consider one of Lambert Instruments image intensifiers, available for various applications.
Several industrial applications such as car crash tests and production line trouble shooting ask for High-Speed Imaging. Combustion research, High Voltage discharge research and some Biological applications do require high-speed imaging. At high frame rate the exposure is short and lighting can become a problem, especially in applications where no artificial lighting can be allowed. We offer image intensifiers designed for high-speed applications that solve this problem. They can easily be attached to an existing high-speed camera to boost the sensitivity.
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LIFA for FLIM with a Nikon microscope and a combined intensity with fluorescence lifetime image. The mammalian cell shows FRET (yellow, 2.2ns) at the centrosomes, the microtubule-organising centers in the cell. The rest of the cell shows no FRET (red, 2,6ns). (Courtesy of A. Diaspro, Genua, Italy) |
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